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The Commission obtained data to analyze average annual GSRs i ; by dispensing channel and ii ; on a drug-by-drug basis for the PBMs' top selling multi-source drugs. 10 Analyses of both sets of data showed that PBM-owned mail-order pharmacies generally had higher average annual GSRs than both retail pharmacies and mail-order pharmacies not owned by a PBM. A. Average Annual Generic Substitution Rates by Channel Showed High Levels of Generic Substitution Across Dispensing Channels and Channel Ownership. Stream of the DRD2 gene and has been repeatedly reported to be associated with, and not associated with, alcoholism meta-analysis in Noble, 1998 ; Hallikainen et al., 2003 ; . The TaqI A RFLP has been associated with heroin use and methadone treatment outcome Lawford et al., 2000 ; , cocaine dependence Noble et al., 1993 ; , psychostimulant abuse Persico et al., 1996 ; , polysubstance abuse O'Hara et al., 1993 ; , and with early age of onset of multiple substance abuse Comings et al., 1994 ; . A DRD2 promoter region variant -141 C ; was associated with heroin use in Chinese subjects Li et al., 2002 ; . When this group was subdivided by route of heroin administration, the association of the promoter variant was of greater significance in the inhaler group, whereas significance was not achieved for the injector group. Another RFLP of DRD2, located in intron 2 TaqI B ; , also was reported to be significantly associated with polysubstance abuse in Caucasians, but not in African Americans O'Hara et al., 1993 ; and in another study with cocaine dependence Noble et al., 1993 ; . In a recent analysis of DRD2 binding in caudate nuclei isolated postmortem from Caucasians, the minor alleles at the TaqI A, TaqI B, and an intron 6 variant were significantly associated with lowered Bmax 40% lower ; of the DRD2 receptor Ritchie and Noble, 2003 ; . In a recent study of six synonymous variants in the DRD2 gene, one of the variants, 957T, was found to cause a decrease in mRNA stability t1 2 8 and a 50% decrease in the translational efficiency of the DRD2 mRNA Duan et al., 2003 ; . This C957T variant altered the predicted DRD2 mRNA folding. Duan and colleagues also found that another naturally occurring variant in DRD2, 1101A, annulled the decrease in mRNA stability conferred by the 957T allele. In addition, it was demonstrated that dopamine stabilized the 957C DRD2 mRNA, whereas there was little stabilization of the 957T DRD2 mRNA. It has been postulated that subjects with reduced DRD2 receptor content may compensate this deficiency through the use of drugs that stimulate the dopaminergic system Noble, 2000 ; . c. Dopamine Transporter Gene SLC6A3 ; . A key regulator of dopaminergic tone is the dopamine transporter, encoded by the gene SLC6A3 see Fig. 1 ; , which regulates the reuptake of dopamine back into the presynaptic neuron, thereby terminating its action. The dopamine transporter is also the major site of action for cocaine's pharmacological actions accounting for cocaine's rewarding properties. A SNP, G2319A, and a variable number of tandem repeats VNTR ; , consisting of a repeat unit of 40 nucleotides, are found in the 3 -untranslated region in exon 15 of the SLC6A3 gene. Subjects with the 9- 10-repeat genotype had a 22% reduction in dopamine transporter availability in the putamen than in subjects homozygous for the 10-repeat allele, as measured by single photon emission computed tomography and plasma radioligand levels, indicating that the VNTR affects expression of.
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